Fluorescent microscopes

Description

The Department of Biology at the University of Copenhagen is based on a legacy of outstanding researchers and is today one of the largest institutes at the University of Copenhagen. The institute has a very unique research and study environment with strong basic research education in biology, biochemistry, molecular biomedicine and bioinformatics. Intended procurement: Purchasing a motorized fluorescence microscope Nikon Ti2-E with environmental control chamber and Photometrics Kinetix camera. Planned use: the microscope will be used in our ongoing projects which are strongly based on the quantitative analysis of cellular, organellar and extracellular pH, Ca2+, and other ion concentrations, in 2D as well as 3D cultures. These projects require rapid, live ratiometric imaging of low-level fluorescence, perfect focus, excellent noise subtraction, a large field of view, long working distance objectives, and complete environmental control. Service context: Acquisition of the microscope, environmental chamber, camera and all accessories.