EVIDENT Microscope Upgrade

Description

The IMPACT (Image Analysis, Multi-Photon and Confocal Technologies) imaging facility provides access to high-end imaging equipment and technical support for research groups in the Centre for Discovery, Brain Science (CDBS) but is also accessible to all research groups across the University. Lot 1: The IMPACT (Image Analysis, Multi-Photon and Confocal Technologies) imaging facility provides access to high-end imaging equipment and technical support for research groups in the Centre for Discovery, Brain Science (CDBS) but is also accessible to all research groups across the University. TIRF (Total Internal Reflection Fluorescence) microscopy is a specialist technique that allows high-contrast, high-resolution images of a broad range of samples. In 2022 the end user purchased a system upgrade for the Evident Scientific TIRF (Total Internal Reflection Fluorescence) microscope which included; Compatibility with existing lasers, objective lenses, filters, camera, TIRF upgrade module, system control and acquisition software and PC. Until now the facility could continue using the original laser source but this has now come to end-of-life. This proposed upgrade will replace the 2-colour laser source with a 4-colour laser source increasing the flexibility and productivity of the microscope. This will further increase the productivity of this system by incorporating high-speed calcium imaging capabilities that allow researchers to examine neuronal signalling.