CellenOne X1

Description

The CellenOne X1 is a unique platform combining high accuracy single-cell isolation and precision nanolitre reagent dispensing, allowing miniaturization and automation of single-cell proteomics protocols using off-the-shelf, commercially available consumables. As the only all-in-one instrument platform developed specifically for such assays, sample loss is kept to an absolute minimum, re-producibility across large batches kept high, and it will provide DTU Bioengineering with unprecedented analytical capabilities in the single-cell proteomics field. Lot 1: DTU Bioengineering is looking to acquire the CellenOne X1 instrument, produced by Cellenion, for expanding its analytical capabilities and consolidating its internationally leading position in the field of single-cell proteomics by Mass Spectrometry (scp-MS). The instrument will be used for running large-scale scp-MS experiments, developing novel microfluidics supported sample preparation workflows, implementing single-cell multiomics experimental workflows and supporting single-cell RNA sequencing experiments. The CellenOne X1 is the only instrument that encompasses all major aspects of scp-MS sample preparation into a single instrument, such as cell isolation, reagent dispensing and maintaining a closely controlled environment for optimal sample preparation conditions. Key features that the CellenOne provides, off-the-shelf and as an integrated instrument platform, and where it sets itself apart from all alternative products are: 1. Multi-fluorescent (blue, green, orange and red) and brightfield image-based cell sorting 2. Lossless cell sorting (collecting ‘discarded’ cells again for re-use) 3. Ultra-gentle dispensing technology 4. Cell depositing in completely controlled x,y,z plane, with axis precision <3µm 5. Dead-volume free cell depositing, i.e. down to 1µl of input sample 6. Automated single-cell proteomics preparation using off-the-shelf consumables 7. Dedicated reagent/consumable workflows for single-cell proteomics (ProteoCHIP, ProteoCHIP LFQ, Nested NanoPOTS) in nL volumes 8. Integrated temperature control, humidity control and dew-point control to prevent evaporation 9. Piezo-acoustic based picolitre to nanolitre dispensing 10. Fully customizable target plate configuration, in all 3 dimensions (x,y,z) 11. Sub-nanolitre volume cell dispensing, enabling extreme assay miniaturization and corresponding dramatic savings in the cost of reagents 12. Droplets are dispensed with microarray-type accuracy and precision with typical spot pitches of down to 100 microns in any pattern and into 96-394- or 1536-well plates or nanowell vessels or onto slides or custom substrates 13. Cells of 2 – 80 microns in diameter can be detected in IBSCITM (bright field) mode, while cells less than 1 micron can be detected using FIBSCITM (fluorescent mode) 14. Images of the droplet containing single, isolated cells and of the cells as they proceed toward the dispensed droplet portion of the nozzle are available for real-time observation and are also preserved for documentation of singularity and review of morphological characteristics. The device utilizes, exclusively, a collection of intellectual property, including that described in the following patents: • Method and apparatus for single particle deposition (20170274689) • Method and dispenser device for depositing an substance on a target substrate (20170072417) • Method and devices for depositing samples on an electrically shielded substrate (8614100) • Spotting plate and process for its production (20130237443) • Microdispenser and associated operating method (20120312836/ 8273307/ 20090060793) • DE1020005025640 (Microdispenser und dazugehoriges Betriebsverfahren) • DE102015011970 (Dispensiererat und entsprechendes Dispensierverfahren) Combined, the CellenOne is the only commercially available sample preparation platform for scp-MS, with no commercially available alternative products on the market at the time of writing.